| AC Connecticut | |
| AHFC Royals | |
| AMSG | |
| Academica | |
| Akron City | |
| Albion Colorado | |
| Almaden | |
| Ann Arbor | |
| Annapolis Blues | |
| Apotheos | |
| Asheville City | |
| Athens United | |
| Ballard | |
| Bangers | |
| Bigfoot | |
| Birmingham Legion II | |
| Black Rock | |
| Blackwatch Rush | |
| Boston Bolts | |
| Boston City | |
| Brazos Valley | |
| Brevard | |
| Brooke House | |
| Buffalo | |
| Capo II | |
| Cedar Stars Rush | |
| Charlotte Eagles | |
| Charlotte Independence 2 | |
| Charlottesville Blues | |
| Chicago Dutch Lions | |
| Christos | |
| City SC | |
| Cleveland Force | |
| Coachella | |
| Colorado ISA | |
| Colorado Storm | |
| Columbus United | |
| Corpus Christi | |
| Davis Legacy | |
| Dayton Dutch Lions | |
| Delaware FC | |
| Denton Diablos | |
| Des Moines Menace | |
| Dothan United | |
| East Atlanta | |
| Erie Sports Center | |
| Evergreen | |
| FC Miami City | |
| FC Motown II | |
| Flatirons Rush | |
| Flint City Bucks | |
| Fort Lauderdale United | |
| Fort Wayne | |
| GFI | |
| Hattiesburg | |
| Hill Country Lobos | |
| Houston FC | |
| Houston Sur | |
| Hudson Valley Hammers | |
| Inter Gainesville | |
| Ironbound | |
| Kalamazoo FC | |
| Kings Hammer | |
| Kings Hammer Columbus | |
| Kings Hammer Sun City | |
| Lane United | |
| Lansing City | |
| Laredo Heat | |
| Lehigh Valley United | |
| Lexington II | |
| Lionsbridge | |
| Little Rock Rangers | |
| Lonestar | |
| Long Island Rough Riders | |
| Louisiana Krewe | |
| Manhattan | |
| Marin | |
| McKinney Chupacabras | |
| Miami | |
| Midlakes United | |
| Midwest United | |
| Minneapolis City | |
| Mississippi Brilla | |
| Monterey Bay II | |
| Montgomery United | |
| Morris Elite | |
| New England FC | |
| New Jersey Copa | |
| Nona | |
| North Carolina Fusion | |
| North Carolina II | |
| Northern Indiana | |
| Oakland County | |
| Ocean City Nor'easters | |
| Oly Town | |
| Patuxent | |
| Peoria | |
| Pinellas County | |
| Project 51O | |
| RKC | |
| Reading United | |
| Real Central New Jersey | |
| Red River | |
| Redlands | |
| River Light | |
| Rochester | |
| SC United Bantams | |
| SF Glens | |
| San Francisco City | |
| San Juan SC | |
| Santafé Wanderers | |
| Sarasota Paradise | |
| Seacoast United Phantoms | |
| Seahorses | |
| Snohomish United | |
| Southern Soccer Academy | |
| Sporting JAX | |
| Springfield | |
| St. Croix | |
| St. Louis Ambush | |
| Stars FC | |
| Staten Island Athletic | |
| Steel City | |
| Sueno | |
| Sunflower State | |
| Tacoma Stars | |
| Tennessee | |
| The Villages | |
| Tobacco Road | |
| Toledo Villa | |
| Tucson | |
| Union Macomb | |
| Utah United | |
| Ventura County Fusion | |
| Vermont Green | |
| Virginia Beach City | |
| Virginia Marauders | |
| Wake | |
| West Chester United | |
| West Seattle Junction | |
| West Virginia Alliance | |
| Westchester Flames | |
| Western Mass Pioneers | |
| Weston | |