| Atlanta United II | |
| Austin II | |
| Carolina Core | |
| Chattanooga | |
| Chicago Fire II | |
| Colorado Rapids II | |
| Columbus Crew II | |
| Crown Legacy | |
| FC Cincinnati II | |
| Houston Dynamo FC II | |
| Huntsville City | |
| Inter Miami II | |
| Los Angeles FC II | |
| Minnesota United II | |
| New England II | |
| New York City II | |
| New York RB II | |
| North Texas | |
| Orlando City II | |
| Philadelphia Union II | |
| Portland Timbers II | |
| Real Monarchs | |
| Sporting KC II | |
| St. Louis City II | |
| Tacoma Defiance | |
| The Town | |
| Toronto II | |
| Vancouver Whitecaps II | |
| Ventura County | |